Puhd10-3

Author: qtam

August undefined, 2024

WebPlasmids used in the experiments. (A) pUBsr9 was constructed so that a bsr gene derived from pSV2- bsr was inserted into pUHD10-3. (B) pTD4 was composed of pUHD10-3 and … WebThe pUHD10-3 vector was digested with EcoRI and BamHI and the cleaved ends were smoothed. The obtained DNA fragment was subcloned into the digested pUHD10-3 …

A possible effector role for the pleckstrin homology (PH) domain …

WebMethods: HLE cells were transfected with pUHD10-3-p53 plasmid contained wt-p53 gene,and treated with DADAG. After 96-hour treatment,apoptosis was evaluated by flow cytometry and DNA electrophoresis. Results: The apoptosis rates were: 1.4% in untreated group, 3.5% in pUHD10-3 ... WebThis project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical … radonova kundtjänst

pTRE-Tight Vector Information - Takara Bio

WebAug 9, 2024 · Faculty of Graduate Studies, University of Calgary. This award was originally endowed in 1989 under the name, “Department of Chemistry Graduate Scholarship.”. In 2013, it was renamed in honour of the department’s long-time graduate program coordinator, Bonnie King, who passed away unexpectedly while still serving in this role. The ... WebPUCH13–3 reporter (luciferase) plasmid, in the presence of increasing amounts of tetracycline (0–5 mg/ml). The selected G418r,tTA1 clonal isolates were used as recipients of 5 mg of PUHD10–3-pX plasmid DNA, co-transfected with an expression vector encoding the hygromycin re-sistance gene. Stable transformants were selected with 200 units/ml WebCognitive and perceptual sciences is one of the featured concentrations of the Department of Psychology. radonpitoisuudet

Reorganization of ErbB Family and Cell Survival Signaling after (2)

Puhd10-3

WebAug 11, 2009 · The large GTPase dynamin plays a key role in clathrin-mediated endocytosis in animal cells, although its mechanism of action remains unclear. Dynamins 1, 2, and 3 … WebFeb 5, 2010 · For analysis of synthesis of murine VEGF, tetracycline (Tet)-regulated TS/A cells stably transfected with pUHD10.3-hGBP-Hyg (cell clones 14 and 18) as well as cells …

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WebAug 11, 2009 · The large GTPase dynamin plays a key role in clathrin-mediated endocytosis in animal cells, although its mechanism of action remains unclear. Dynamins 1, 2, and 3 contain a pleckstrin homology (PH) domain that binds phosphoinositides with a very low affinity (K D > 1 mM), and this interaction appears to be crucial for function. These … WebConstruction of Xenopus (B3.2) and human (HeLa) cell lines expressing the tetracycline-controlled transactivator (tTA)

Webpromoter in pUHD10-3 vector (28) provided by H. Bujard (Heidelberg University, Heidelberg). The pUHD10-3 puro vec-tor was constructed by ligating puromycin … WebJul 28, 2010 · pUHD10-3 in reference 5. The pTRE-Tight-Luc Control Vector, packaged with the pTRE-Tight Vector, contains an ad-ditional 1,649 bp encoding firefly luciferase …

WebThe coding sequence of hGBP-1 was isolated from pMCV1.4 + GBP-1 (25) by restriction digestion with EcoRI and inserted into the linearized tetracycline response plasmid … WebMethods: HLE cells were transfected with pUHD10-3-p53 plasmid contained wt-p53 gene,and treated with DADAG. After 96-hour treatment,apoptosis was evaluated by flow …

WebJun 11, 2024 · Wild type beta-catenin cDNA containing the entire cording region and a mutated form of beta-catenin cDNA lacking exon3 including specific GSK-3beta phosphorylation sites were cloned into pUHD10-3 vector under regulation of a tetracycline-responsive promoter.These cDNAs was each cotransfected with pUHD15-1Neo, and …

WebPhCMV*-1 region in plasmid pUHD10-3, but the tetO sequences were retained. The E2a ORF, with its endog-enous early promoter (E-P) and late promoter (L-P) cloned from … radonputki eristysWeba pUHD10-3 hygromycin vector (Reynisdottir et al., 1995). We were able to identify at least six clones (6/ 10), and one representative clone can express p27 in the radonputken asennusWebFeb 27, 2004 · Generation of Cells Responsive to Inducible Dimerization of FADD— The plasmid pUHD10-3-FKBP 2-link-FADD carries the coding sequence for a myristoylation-targeting peptide from the amino terminus of v-src, two tandem repeats of FKBP12, a short linker peptide, the human FADD cDNA, and the influenza HA epitope (Fig. 1A).The … radonpotentialklassenWebSep 16, 1996 · We have underexpressed the 43 kDa 5-phosphatase by stably transfecting normal rat kidney cells with the cDNA encoding the enzyme, cloned in the antisense … cva national advisory committeeWebAug 1, 1997 · The plasmids of the original Tc-regulatable expression system, pUHD15-1 (ptTA), pUHD10-3 (pTetP) and pUHC13-3 (pTetP-Luc), were provided by Dr H.Bujard . … radonpurkkiWebway, operative when the regulated pathway is `saturated' [3]. To study this issue, we used a system that is widely em-ployed to regulate protein synthesis in cultured cells and in transgenic mice by varying the corresponding mRNA synthe-sis [4]. This regulatory system exploits the sensitivity of mam- cva nettingWebNov 1, 1999 · The inducible mammalian cell expression vector pUHD10-3 , which contains a Tet-suppressible (Tet-off) promoter, was used to generate two sets of PTEN expression … radonpitoisuus