WebThe porcine ear cartilage was covered with perichondrium and cellular constituents were found to be composed of chondrocytes and chondroblasts. In addition, the extracellular matrices were mainly composed of collagen. Upon xenograft of irradiated cartilage into rats, there was no specific inflammatory reaction around the transplanted cartilage. WebNov 9, 2012 · Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for biomedical research. However, the efficiency of this approach is crucially dependent on the source of nuclear donor cells. In this study, we evaluate the potential of primary porcine kidney cells (PKCs) as cell source for …
Porcine ear skin: an in vitro model for human skin - PubMed
WebFibroblasts isolated from ear skin biopsies of these piglets were cultured for 6 to 8 passages to induce loss of heterozygosity (LOH) and treated with biotin-conjugated IB4 that binds to galactose-α-1,3-galactose, an epitope produced by αGT. Using magnetic activated cell sorting, cells with monoallelic disruption of αGT were removed. WebPorcine skin has a high similarity to human skin, and thus, is often used as a model for human skin [1,2,3,4,5].The ex vivo porcine ear model has various applications; in most … datuk clifford hii
Evaluating the Effects of Cryopreservation on the Viability and …
WebSep 26, 2012 · Recently, an important causative role has also been attributed to immunosuppressive agents such as Porcine Circovirus type 2 (PCV2), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), as well as mycotoxins. 7,13 PCV2 is considered to be involved in etiology agents of the development of PNES 14 and is present in ear lesions … WebPorcine necrotic ear syndrome (PNES) in pigs has been reported as an increasing health problem in many countries with intensive pig farming. The etiology of this disease is … WebSep 13, 2024 · Extracellular matrix (ECM) hydrogels provide advantages such as injectability, the ability to fill an irregularly shaped space, and the adequate bioactivity of native matrix. In this study, we developed decellularized cartilage ECM (dcECM) hydrogels from porcine ears innovatively via the main method of enzymatic digestion and verified … datuk che mohamad zulkifly jusoh