L34966 thermo
WebAug 19, 2024 · Live/Dead Aqua (catalog L34966, Thermo Fisher Scientific) was used to assess cell viability. NK cells were gated as follows: live CD3 – CD19 – B220 – CD45 + NKp46 + CD49b +. ELISA. sLeA secretion from transduced tumor cells was detected by sandwich ELISA. WebGloCell™ Fixable Viability Dyes Increase the Accuracy of Flow Cytometry Data by Excluding Dead Cells GloCell™ Fixable Viability Dyes are fluorescent amine-labeling dyes for staining of live/dead mammalian cells, allowing clear exclusion of …
L34966 thermo
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WebHomes in ZIP code 29016 were primarily built in the 2000s. Looking at 29016 real estate data, the median home value of $209,300 is slightly higher than average compared to the … Web400 (L34966) 405 nm 367 nm 526 nm Yellow fluorescent reactive dye 80 (L34967) 200 (L34959) 400 (L34968) 405 nm 400 nm 575 nm Green fluorescent reactive dye 80 …
WebApr 15, 2024 · Cells were stained using Live/Dead Blue (Invitrogen, cat no. L34962) or Live/Dead Aqua (Invitrogen, cat no. L34966) first and then incubated with human FcR blocking reagent (Miltenyi Biotec) and ... WebMay 1, 2024 · Cells were treated with a live/dead stain (L34966, Thermo) as per manufacturer’s instructions. Cells were then fixed with 4% paraformaldehyde for 15 min at RT. The same staining protocol as described for ICC was followed, using an anti-TYRP1 primary antibody and run on the ThermoFisher Attune NxT flow cytometer. Data was …
WebMay 10, 2024 · Cells were stained using LIVE/DEAD® Fixable Aqua Dead Cell Stain (Thermo Fisher Scientific cat # L34966) following the manufacturer’s instructions and then resuspended in Hank’s Balanced Salt Solution (HBSS) (Thermo Fisher Scientific cat # 14025076). Cell populations were gated on the BD FACSAria™ Fusion Sorter based on … WebInvitrogen™ LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation Catalog number: L34966 Related applications: Cell Viability, Proliferation & Function Technical …
Web400 (L34966) 405 nm or UV 367 nm 526 nm Yellow fluorescent reactive dye 80 (L34967) 400 (L34968) 405 nm 400 nm 575 nm Green fluorescent reactive dye 80 (L34969) 400 …
WebMay 5, 2024 · This protocol can be used for isolating and analyzing tissue-resident immune cells from other tissues with specified modifications. For complete details on the use and execution of this protocol, please refer to Kim et al. (2024). Highlights Optimized protocol to isolate and analyze mouse intestinal lamina propria lymphocytes solar carvings grasshopperWebOct 1, 2024 · Fixable live/dead cell discrimination was performed using LIVE/DEAD Fixable Aqua stain (Thermo Fisher Scientific, Catalog No. L34966) according to the manufacturer's instructions. Surface staining was carried out on ice for 30 minutes if not indicated otherwise, and intracellular staining was performed using the Foxp3 staining kit … solar carport hotelsWebBrea, CA), a CytoMat2 incubator (Thermo Fischer Scientific, Waltham, MA), a Vspin centrifuge (Agilent Technologies, Santa Clara, CA), an ELx405 cell washer (BioTek, 5 ... (ThermoFisher, Cat# L34966), fixed and permeabilized, resuspended and analyzed on iQue Screener Plus using the same protocol as previously described for Treg cells. The … slumberland furniture hibbingWebProduct Specs Item LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation Company Thermo Fisher Scientific Price Pricing Info Supplier Page View Company … solar car parking shedWebCells were stained for 30–60 min in the dark on ice, then cells were washed with PBS and resuspended in 0.5% BSA and 2 mM EDTA pH 8.0, in 1× PBS for sorting or flow cytometry analysis as previously descri bed4,16,20,22,25. For astrocyte surface marker screening, CNS cells derived from Tg(Aldh1l1-EGFP,-DTA)D8Rth/J mice were plated on LegendScreen … slumberland furniture hibbing mnWebLIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation 400 assays L34966 LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation 400 assays L34968 LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation 400 assays L34976 PrestoBlue™ HS Cell Viability Reagent 100 mL P50201 slumberland furniture home officeWebManufacturer: LIFE TECHNOLOGIES L34966 Catalog No. 50-112-1526 $356.00 / Each of 1 Qty Check Availability Add to cart Description Documents Customers who viewed this … slumberland furniture hibbing minnesota