Hitrap sp hp 5 ml manual
WebThe recommended flow rate for HiTrap Heparin HP is 0.1 to 1 ml/min or 1 to 5 ml/min for 1 ml or 5 ml column respectively. 1 Fill the syringe or pump tubing with binding buffer. Remove the stopper and connect the column to the syringe (with the provided connector), or pump tubing, “drop to drop” to avoid introducing air into the column. Webユーザーズマニュアル GE SP HP の仕様概要. ページ 1 GE Healthcare Instructions 71-7149-00 AN HiTrap ion exchange columns HiTrap SP HP, 1 ml and 5 ml HiTrap Q …
Hitrap sp hp 5 ml manual
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WebRemove the snap-off end at the column outlet. Equilibrate the column with 5 column volumes of binding buffer. Apply the pretreated sample using a syringe fitted to the Luer connector or by pumping it onto the column. For best results, use a flow rate of 0.2 to 1 mL/min (1 mL column) and 0.5 to 5 mL/min (5 mL column) during sample application 1. WebProtein load was increased five-fold for the scale-up from the 1 mL StrepTrap™ HP column to the 5 mL column and 25-fold in the scale-up from the 1 mL StrepTrap™ HP column to the 29 mL XK 26/20 column. Yield, calculated from absorbance measurements, was 2.2, 9.4, and 52.7 mg, respectively (Table 7.1).
WebCytiva 29-0513-24, pack of 1 mL. All Photos (3) NACRES: NA.56. Recommended Products. Slide 1 of 1. 1 of 1. OLIGO. ... HiTrap SP HP are prepacked, ready-to-use SP Sepharose High Performance strong cation exchange columns for … Web27 feb 2024 · 上海易汇生物科技有限公司供应GE总代理-GE总代 GE全国销售代理 GE上海代理供应产品,GE全国理GE销售 GE上海Product Part # Description 2024 China LLP 18100062 18100062 QUARTZ GLASS ASSEMBLY, 2 MM PATH LE 7,173 18100063 18100063 QUARTZ GLASS ASSEMBLY, 10 MM PATH L 7,173 18100064 18100064
WebHiTrap Desalting columns (prepacked with Sephadex G-25) enable fast sample clean up in less than 5 minutes for sample volumes from 0.25 to 1.5 ml, as shown in Figure 6. To increase the maximum sample volume capacity to 3 ml simply connect two columns in series. HiTrap Desalting columns are ideal for desalting, buffer exchange, and removal … http://www.user-manual.info/245515/dishwasher/ge/hitrap-sp-hp/
WebHiTrap™ SP HP, 1 ml and 5 ml HiTrap Q HP, 1 ml and 5 ml HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method …
WebColumn volume (CV) 1 ml 5 ml Column dimensions 0.7 × 2.5 cm 1.6 x 2.5 cm Column hardware pressure limit 5 bar (0.5 MPa) 5 bar (0.5 MPa) Maximum flow rates1 4 ml/min, 20 ml/min Recommended flow rates 1ml/min 5 ml/min Connectors supplied Usage No. supplied Union 1/16” male/ luer female For connection of syringe to HiTrap column 1 … the washington times media biasWeb17-5248-05 HisTrap HP 5 ml 100 × 5 ml* * Pack size available by special order. Connector kit Connectors supplied ... Stop plug female, 1/16” Sealing bottom of HiTrap column 2, 5 … the washington times media bias fact checkWebMax. flow rate* 4 ml/min (1 ml), 20 ml/min (5 ml) Recommended Flow rate 1 ml/min (1 ml), 5 ml/min (5 ml) pH stability** Working 5 to 10 Cleaning 5 to 10 Temperature stability Working 4°C to room temp. Storage 4°C to 30°C Storage 20% ethanol * H 2 O at room temperature ** The ranges given are estimates based on our knowledge and experience. the washington times llcWebSP HP - read user manual online or download in PDF format. Pages in total: 20. SP HP - read user manual online or download in PDF format. Pages in total: 20. … the washington times logoWeb16 nov 2012 · HiTrap SP HP, 1 ml and 5 ml. HiTrap Q HP, 1 ml and 5 ml. HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion. exchange columns for … the washington times 1999 newspaperWebcomponents elute between 1.5 and 4.5 ml, depending on the sample volume. Low molecular weight components start to elute after 3.5 ml. See Figure 3 for the effect of varying the … the washington times vacation holdWebHiTrap SP XL are strong cation exchangers prepacked with SP Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange ... 5 × 5 … the washington times newspaper online